yabO [2019-08-15 13:04:57]

similar to heat shock protein Hsp15

Locus: BSU_00590

Isoelectric point: 10.14

Molecular weight: 9.58 kDa

Protein length: 86 aa

Gene length: 261 bp

Function: recycling of stalled ribosomes

Product: Hsp15

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Translation/ other/ based on similarity

Gene

Coordinates: 67,877 → 68,137

The protein

Catalyzed reaction/ biological activity

• translocates the tRNA moiety from the A site to the P site of stalled 50S ribosomal subunits PubMed

Protein family

• S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)

Domains

• S4 RNA-binding domain (aa 1 ... 63) (according to UniProt)

Structure

• 3BBU (from E. coli, 36% identity) PubMed

Expression and Regulation

Operons

• Genes: yabM-yabN-yabO-yabP-yabQ-divIC-yabR
  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigE: sigma factor, PubMed, in SigE regulon
  • SigW: sigma factor, PubMed, in SigW regulon
  • SigD: sigma factor, PubMed, in SigD regulon
  • SigX: sigma factor, PubMed, in SigX regulon

  Regulation

  • expressed during sporulation (SigE) PubMed

Biological materials

Mutant

• MGNA-B916 (yabO::erm), available at the NBRP B. subtilis, Japan
• BKE00590 (ΔyabO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAATTTATCTAATCTCA, downstream forward: _UP4_TAGGCTTGTTCTAAAAAAAC
• BKK00590 (ΔyabO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAATTTATCTAATCTCA, downstream forward: _UP4_TAGGCTTGTTCTAAAAAAAC

References

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